Recapitulate the initiation and progression of Barrett’s oesophagus using patient-derived organoids

Principal Investigators: Dr John Lizhe Zhuang and Dr Alvin Ng, University of Cambridge.

Funded by: CRUK Early Detection Primer Award 2021

Oesophageal adenocarcinoma (OAC) has increased almost 6-fold in western countries in the past three decades, with a very poor prognosis of 15% five-year survival rate. OAC originates from a precursor lesion, known as Barrett’s oesophagus (BO), which bears a high mutation burden that is greater than many invasive cancer types. In this project, we have developed a number of BO organoid lines that derived from patients which serve as a comprehensive in-vitro platform to study the cancer and its precancerous lesion, and dissect the genomic heterogeneity and the malignant transformation. This understanding will aid accurate early detection and risk stratification of BO/OAC patients.