Deciphering the immune microenvironment in oesophageal adenocarcinoma and pre-cancerous lesions to a single cell resolution

Principal Investigators: Dr John Lizhe Zhuang (CRUK) & Dr Young Hwan Chang (OHSU)

Funded by: OHSU-CRUK SPARK Award 2018

Dr John Lizhe Zhuang (Fitzgerald Lab) and Dr Young Hwan Chang (OHSU) have been awarded funding for their project ‘Deciphering the immune microenvironment in oesophageal adenocarcinoma and pre-cancerous lesions to a single cell resolution’.

The project aims for a comprehensive characterisation of immune complexity of oesophageal adenocarcinoma using a novel sequential multiplex immunohistochemistry technique combined with computational image analysis. The method allows us to study the dynamic changes of immune populations along the progression of the disease from metaplasia to cancer, as well as the immune association with different tumour mutation signatures. In addition, using a machine learning algorithm, we will deep analyse the multi-parametric data on a single cell basis and look for more specific identification of cell subsets. The study will shed a light on an unclear field in oesophageal adenocarcinoma and better facilitate patient stratification, therapy and prognosis.